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KMID : 0364819880260020093
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Korean Journal of Microbiology
1988 Volume.26 No. 2 p.93 ~ p.100
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Isolation and characterization of glutamate dehydrogenase defective mutant of Brevibacterium flavum
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Choi, Soon Young/최순영
Sung, Ha Chin/Min, Kyung Hee/성하진/민경희
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Abstract
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1
In order to understand the regulation of glutamate dehydrogenase(GDH) synthesis in Brevibacterwm flavwn, we have isolated a mutant lacking NADP-linked GDH activity by ethylme thane salfoaate treatment. The gdh- mutant was grown on the minimal plate with 1mM ammonium chloride and not that with 300mM ammonium chloride. The cell-free extracts from gdh- mutant and prototroph were also examined with glutamine synthetase(GS) and glutamate synthase(GOGAT) production by nitrogen sources. The growth of gdh- mutant in presence of 20mM ammonium chloride mans that GOGAT synthesis is sufficient to allow growth in this condition. GS production of gdh- mutant as well as parental strain was induced by 1mM urea and ammonium tartrate, but it was repressed by higher concentration of ammonia, and also induced by 20mM to SOmM glutamate as a substrate. It was special attention that GOGAT synthesis from gdh- strain was more repressed by higher concentration of ammonia than prototroph as described in E. coil system.
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KEYWORD
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